Detection of genes mutations in cerebrospinal fluid circulating tumor DNA from neoplastic meningitis patients using next generation sequencing
نویسندگان
چکیده
منابع مشابه
Next-Generation Sequencing of Circulating Tumor DNA for Early Cancer Detection
Curative therapies are most successful when cancer is diagnosed and treated at an early stage. We advocate that technological advances in next-generation sequencing of circulating, tumor-derived nucleic acids hold promise for addressing the challenge of developing safe and effective cancer screening tests.
متن کاملDetection of mitochondrial DNA mutations in the tumor and cerebrospinal fluid of medulloblastoma patients.
Medulloblastoma is the most common malignant brain tumor in children. Although the prognosis has improved considerably, leptomeningeal spread of the tumor remains a significantly negative predictor of survival. Mitochondrial DNA (mtDNA) mutations have been detected in many types of human tumors but not in medulloblastomas. Using temporal temperature gradient gel electrophoresis, we have analyze...
متن کاملCEREBROSPINAL FLUID FINDINGS IN NEONATAL BACTERIAL MENINGITIS
Background : Meningitis is more common during the neonatal period than other periods of life and has a prevalence of one in 1000 live births. The similarity of its clinical manifestations with other infectious diseases makes it difficult to diagnose. The aim of the current study was to determine the relationship between age, sex, clinical signs and symptoms and laboratory test results of ne...
متن کاملDETECTION OF HAEMOPHILUS INFLUENZAE TYPE B IN CEREBROSPINAL FLUID OF SUSPECTED CHILDREN WITH MENINGITIS BYPCR
ABSTRACT Background: A polymerase chain reaction (PCR) assay with primers from "hpd" gene was compared with culture for the detection of Haemophilus influenzae type b (Hib) in CSF samples from children suspected of meningitis. Methods: 300 cerebrospinal fluid (CSF) samples from suspected meningitis cases were studied by culture (Leventhal & Chocolate Agar) and PCR. Results: The latter...
متن کاملDetection of Therapeutically Targetable Driver and Resistance Mutations in Lung Cancer Patients by Next-Generation Sequencing of Cell-Free Circulating Tumor DNA.
PURPOSE The expanding number of targeted therapeutics for non-small cell lung cancer (NSCLC) necessitates real-time tumor genotyping, yet tissue biopsies are difficult to perform serially and often yield inadequate DNA for next-generation sequencing (NGS). We evaluated the feasibility of using cell-free circulating tumor DNA (ctDNA) NGS as a complement or alternative to tissue NGS. EXPERIMENT...
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ژورنال
عنوان ژورنال: BMC Cancer
سال: 2020
ISSN: 1471-2407
DOI: 10.1186/s12885-020-07172-x